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total histone h3  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc total histone h3
Western blot analysis of histone <t>H3</t> and H4 acetylation in urothelial cancer cell lines after HDAC8 knockdown and HDAC8 inhibitor treatment. Amounts <t>of</t> <t>acetylated</t> and total histone H3 and H4 were analyzed by western blotting. The modifications are determined in the urothelial cancer cell lines RT-112, VM-CUB1, SW-1710, 639-V and UM-UC-3 after (A) siRNA mediated HDAC 8 knockdown (72 h) in comparison to untreated and irrelevant control and (B) pharmacological HDAC8 treatment (compound 2, compound 5, compound 6; IC 50 , 72 h). DMSO served as a solvent control.
Total Histone H3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Western blot analysis of histone H3 and H4 acetylation in urothelial cancer cell lines after HDAC8 knockdown and HDAC8 inhibitor treatment. Amounts of acetylated and total histone H3 and H4 were analyzed by western blotting. The modifications are determined in the urothelial cancer cell lines RT-112, VM-CUB1, SW-1710, 639-V and UM-UC-3 after (A) siRNA mediated HDAC 8 knockdown (72 h) in comparison to untreated and irrelevant control and (B) pharmacological HDAC8 treatment (compound 2, compound 5, compound 6; IC 50 , 72 h). DMSO served as a solvent control.

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Histone deacetylase 8 is deregulated in urothelial cancer but not a target for efficient treatment

doi: 10.1186/s13046-014-0059-8

Figure Lengend Snippet: Western blot analysis of histone H3 and H4 acetylation in urothelial cancer cell lines after HDAC8 knockdown and HDAC8 inhibitor treatment. Amounts of acetylated and total histone H3 and H4 were analyzed by western blotting. The modifications are determined in the urothelial cancer cell lines RT-112, VM-CUB1, SW-1710, 639-V and UM-UC-3 after (A) siRNA mediated HDAC 8 knockdown (72 h) in comparison to untreated and irrelevant control and (B) pharmacological HDAC8 treatment (compound 2, compound 5, compound 6; IC 50 , 72 h). DMSO served as a solvent control.

Article Snippet: The detection of acetylated and non-acetylated histones was performed with primary antibodies against acetylated histone H3 (1:2,000, #39139, Active Motif, La Hulpe, Belgium), total histone H3 (1:1,000, #3638, Cell Signaling Technology, Inc., Danvers, MA), acetylated histone H4 (1:1,000, #39243, Active Motif, La Hulpe, Belgium) and total histone H4 (1:500, #39269, Active Motif, La Hulpe, Belgium).

Techniques: Western Blot